Cell Biology Group
Renata Dmitrieva, PhD in BiologyHead of the Group
The projects of the Cell Biology Group are complex multidisciplinary studies involving a wide range of specialists: from the laboratories of the Institute of Molecular Biology and Genetics, Heart Failure Research Laboratory, bioinformaticians of the Genomic Diversity Centre of ITMO University, the Laboratory of Myology of the Institute of Biomedical Problems of the Russian Academy of Sciences, the Department of Human and Animal Physiology of Lomonosov Moscow State University.
Major research areas
- Study of the mechanisms of impaired skeletal muscle regeneration and metabolism in cardiovascular diseases, including chronic heart failure and laminopathies
- Development of cellular models of skeletal muscle dystrophy, search for therapeutic targets for the prevention and treatment of muscle degeneration
- Bioinformatics analysis of data obtained in biological experiments.
- Study of the biophysical characteristics of voltage-gated ion channels in cardiomyocytes by local voltage clamping.
Participation in State Assignments
- “The role of epigenetic mechanisms in the regulation of the regenerative potential of organs and tissues in cardiometabolic syndrome and heart failure” (2015 – 2017, supervised by R. Dmitrieva)
- “Study of the regulation mechanisms of the voltage-gated sodium channel Nav1.5 to search for new targets for treating arrhythmias” (2018 – 2020, supervised by A. Karpusheva)
Grants
Grant of the Russian Science Foundation 16-15-10178
“Study of the mechanisms of impaired skeletal muscle regeneration and pathological replacement of functional muscle tissue with adipose tissue” (R. Dmitrieva, 2016–2020)
Grant of the Russian Science Foundation 17-15-01292
“Structural and molecular mechanisms of dysfunctional Nav1.5 channel in the myocardium” (B. Zhorov, 2017–2021)
Grant of the Russian Science Foundation 20-75-10080
“Molecular basis of the regenerative potential of soleus muscle satellite cells at different stages during functional unloading” (N. Vilchinskaya, 2020–2023, jointly with the Laboratory of Myology of the Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow)
The following specialists of the Institute of Molecular Biology and Genetics are involved in grants together with the specialists of the Cell Biology Group:
- Natalya Khromova, Researcher, Laboratory of Molecular Cardiology and Genetics
- Elena Ignatieva, Junior Researcher, Laboratory of Molecular Cardiology and Genetics
Training and education
The specialists of the Group are actively involved in the educational projects of the Centre:
- Dmitrieva and A. Karpushev are associate professors at the Department of Biology, the Faculty of Biomedical Sciences of the Institute of Medical Education.
- In September 2020, O. Ivanova, R. Dmitrieva and A. Karpushev prepared and delivered educational modules “Approaches to Transcriptome Analysis in a Biological Experiment” and “Cell Electrophysiology” at Sirius University.
Projects
- Study of the impaired functional properties of skeletal muscle stem cells in chronic heart failure
- Study of the impaired development and metabolism of skeletal muscles in laminopathies
- Devising cellular models of skeletal muscle degeneration, search for therapeutic targets for the prevention and treatment of muscle degeneration
- Rerio as a model object in studies of cardiac channelopathies
- Regulation of voltage-gated ion channels by small G-proteins
- Structural and functional characteristics of the cardiac isoform of sodium channels with amino acid substitutions associated with arrhythmogenic syndromes
Ignatieva EV, Ivanova OA, Komarova MY, Khromova NV, Polev DE, Kostareva AA, Sergushichev AA, Dmitrieva RI. LMNA Mutations G232E and R482L Cause Dysregulation of Skeletal Muscle Differentiation, Bioenergetics, and Metabolic Gene Expression Profile. 2020, Genes 11 (9), 1057. Access link: https://www.mdpi.com/2073—4425/11/9/1057.
Lelyavina T.A., Galenko V.L., Ivanova O.A., Komarova M.Yu., Ignateva E.V., Bortsova M.A., Khromova N.V., Sitnikova M.Yu., Kostareva A.A., Dmitrieva R.I., Sergushichev A., Yukina G.Y. Сlinical response to personalized exercise therapy in heart failure patients with reduced ejection fraction is accompanied by skeletal muscle histological alterations. International Journal of Molecular Sciences. 2019. Т. 20. № 21. С. 5514. IF 4,556. Access link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6862491/.
Dmitrieva R.I., Khromova N.V., Golovkin A.S., Kostareva A.A., Lelyavina T.A., Galenko V.L., Bortsova M.A., Sitnikova M.Yu., Komarova M.Yu., Ivanova O.A., Sergushichev A., Tikanova P.A. Skeletal muscle resident progenitor cells coexpress mesenchymal and myogenic markers and are not affected by chronic heart failure-induced dysregulations. Stem Cells International. 2019. Т. 2019. С. 5690345. IF 3,902. Access link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335669/.
Kiselev A.M., Vaz R., Knyazeva A., Sergushichev A., Dmitrieva R.I., Khudyakov A.A., Jorholt J., Smolina N.A., Sukhareva K., Fomicheva Yu., Mikhailov E.N., Mitrofanova L.B., Predeus A., Sjoberg G., Rudenko D.I., Sejersen T., Lindstrand A., Kostareva A.A. Truncating variant in myof gene is associated with limb-girdle type muscular dystrophy and cardiomyopathy. Frontiers in genetics. 2019. Т. 10. № JUN. С. 608. IF 3,789. Access link: https://pubmed.ncbi.nlm.nih.gov/31297131/.
Zaytseva A.K., Karpushev A.V., Kiselev A.M., Mikhaylov E.N., Lebedev D.S., Zhorov B.S., Kostareva A.A. Characterization of a novel scn5a genetic variant a1294g associated with mixed clinical phenotype. Biochemical and Biophysical Research Communications. 2019. Т. 516. № 3. С. 777-783. IF 2,985. Access link: https://pubmed.ncbi.nlm.nih.gov/31253402/.
Tarnovskaya S.I., Korkosh V.S., Zhorov B.S., Frishman D. Predicting novel disease mutations in the cardiac sodium channel. Biochemical and Biophysical Research Communications. 2020. Т. 521. № 3. С. 603-611. IF 2,985. Access link: https://www.sciencedirect.com/science/article/pii/S0006291X19320509.
Dmitrieva R.I., Cranford S.M., Doris P.A. Genetic control of serum marinobufagenin in the spontaneously hypertensive rat and the relationship to blood pressure. Journal of the American Heart Association. 2017. Т. 6. № 10. С. e006704. IF 2,705. Access link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5721872/.
Popova P.V., Vasileva L.B., Tkachuk A.S., Puzanov M.V., Bolotko Y.A., Pustozerov E.A., Gerasimov A.S., Zazerskaya I.E., Li O.A., Vasileva E.Yu., Kostareva A.A., Dmitrieva R.I., Grineva E.N. Association of tribbles homologue 1 gene expression in human umbilical vein endothelial cells with duration of intrauterine exposure to hyperglycaemia. Genetical Research. 2018. Т. 100. С. e3. IF 2,53. Access link: https://pubmed.ncbi.nlm.nih.gov/29502537/.
Perepelina K.I., Dmitrieva R.I., Ignateva E.V., Borodkina A.V., Kostareva A.A., Malashicheva A.B. Lamin A/C mutation associated with lipodystrophy influences adipogenic differentiation of stem cells through interaction with notch signaling. Biochemistry and Cell Biology. 2018. Т. 96. № 3. С. 342-348. IF 2,46. Access link: https://pubmed.ncbi.nlm.nih.gov/29040816/.
Dmitrieva R. I., Minullina I. R., Bilibina A. A., Tarasova O. V., Anisimov S. V., Zaritskey A. Y.
Bone marrow- and subcutaneous adipose tissue-derived mesenchymal stem cells: Differences and similarities. Cell Cycle. 2012 Jan 15; 11 (2): 377-83.
R. I. Dmitrieva, C. A. Hinojos, M. L. Grove, R. J. Bell, E. Boerwinkle, M. Fornage, P. A. Doris.
Genome-Wide Identification of Allelic Expression in Hypertensive Rats Circ Cardiovasc Genet. 2009; 2: 106-115.
Dmitrieva R. I., Hinojos C. A., Boerwinkle E., Braun M. C., Fornage M., Doris P.A.
Hepatocyte nuclear factor 1 and hypertensive nephropathy. Hypertension. 2008 Jun; 51 (6): 1583-9.
Tian D., Dmitrieva R. I., Doris P. A., Crary J. F., Sondhi R., Sacktor T. C., Bergold P. J.
Protein kinase M zeta regulation of Na/K ATPase: a persistent neuroprotective mechanism of ischemic preconditioning in hippocampal slice cultures. Brain Res. 2008 Jun 5; 1213: 127-39.
Karpushev A. V., Levchenko V., Ilatovskaya D. V., Pavlov T. S., Staruschenko A.
Novel role of Rac1/WAVE signaling mechanism in regulation of the epithelial Na+ channel. Hypertension. 2011; 57 (5): 996-1002.
Karpushev A. V., Vachugova D. V., Pavlov T. S., Negulyaev Y. A., Staruschenko A. Intact cytoskeleton is required for small G protein dependent activation of the epithelial Na+ channel. PLoS ONE. 2010; 5 (1): e8827.
Pochynyuk O., Kucher V., Boiko N., Mironova E., Staruschenko A., Karpushev A. V., Tong Q., Hendron E., Stockand J.
Intrinsic voltage dependence of the epithelial Na+ channel is masked by a conserved transmembrane domain tryptophan. J Biol Chem. 2009; 284 (38): 25512-21
Karpushev A. V., Ilatovskaya D. V., Staruschenko A.
The actin cytoskeleton and small protein RhoA are not involved in flow-dependent activation of ENaC. BMC Res Notes. 2010; 27 (3): 210.
Karpushev A. V., Levchenko V., Pavlov T. S., Lam V. Y., Vinnakota K. C., Vandewalle A., Wakatsuki T., Staruschenko A.
Regulation of ENaC expression at the cell surface by Rab11. Biochem Biophys Res Commun. 2008; 377 (2): 521-5.